at Saint Louis University
School of Medicine

Email Us ||   Services Fees ||   Service and Fee Flowchart ||   Schedule Overview

To start a project or for more information call 314-977-8890
or contact us at: ANTIBODY@SLU.EDU or try our e-mail form.

• We handle all hybridoma technology procedures from mice injections to final subcloning, cell line expansion and antibody production.
• All you will need to do is to supply 1 to 5 mg of antigen and confirm that the hybridoma supernatants that we identify in our proprietary dot blot screening procedure contains antigen-specific monoclonal antibody reactivities.
• Hybridoma cell lines and monoclonal antibodies are your exclusive property.
• All hybridoma procedures are scheduled using computerized tracking software.
• We will coordinate in advance with your investigators on what days they will need to confirm antibody specificities
• Projects performed by GLP/cGMP standards.

Startup and Immunization Phase Fee	$2500
Fusion Phase Fee	$2500
Subcloning Phase Fee	$2500
Total	$7500

Additional hybridoma service Fees
Third or additional fusions	$2500
Subcloning of an additional cell line	$ 500
Mouse housing charges	$ 50 per month
Two additional immune boosts and screening	$1000
Expansion of an additional cell line to generate 20 mls of hybriodma supernatant	$ 500
Immunization phase done on reordered mice	$1000
Dot blot rescreening on up to	$ 500 per 12 samples
Western strip blotting secondary screening	$1000 per 12 samples
Immunoprecipitation secondary screening	$1000 per 12 samples
In-Vitro Monoclonal Antibody Production Service Fees.
Integra Celline CL-1000 Harvest 50 ml volume.	$1000
Integra Celline CL-350 Harvest 15 ml volume.	$ 500

Discounts are available on multi-projects beginning at the same time.
Please call for specific information.

Prices effective December 1,2002

An Overview of Our Hybridoma Development Service Schedule

Day -2	Prebleed each mouse.
Day 1	Immunize mice with antigen mixed with complete Freund's adjuvant .
Day 14	Immunize mice with antigen mixed with incomplete Freund's adjuvant.
Day 21	Collect immune bleeds.
Day 28	Immunize mice with antigen mixed with incomplete Freund's adjuvant.
Day 35	Collect immune bleeds.
Day 35	Send investigator 10 to 50 µl of each immune serum. The investigator needs to have the immune serum analyzed during the next 2 days and have results ready to contribute to the consultation on Day 38.
Day 36-37	Our facility will independently monitor the immune bleeds for antigen-specific reactivities.
Day 38	Consult with the investigator and compare immune screening results. Decide which immune-responsive mice will be used for fusion(s). Your first fusion will be scheduled in the first available date, usually with two weeks. Starting from the day the fusion is done, the following is a representative schedule.
Day -3	Perform the fusion boost injection on a selected mouse.
Day 1	Perform hybridoma fusion technology
Day 14	Screen all hybridoma supernatants. Send investigator up to 20 putative positive supernatants for confirmation.* The investigator needs to have these supernatants analyzed during the next 2 days and decide which of up to 4 hybridoma populations should be subcloned. If no positives are identified we will schedule a second fusion.
Day 17	Subclone up to 4 positive hybridoma cell populations.
Day 35	Screen subcloned hybridoma supernatants. Send investigator up to 20 putative positive supernatants from subcloned hybridoma cell lines for confirmation. The investigator needs to have these supernatants analyzed during the next 2 days and decide which 2 hybridoma cell lines should be used in a second round of subcloning.
Day 38	Second round subcloning on 2 of the hybridoma cell lines.
Day 52	Screen the twice subcloned hybridoma cell lines. Send investigator supernatants from twice subcloned hybridoma cell lines for confirmation. Expand cell lines.
Day 70	Send supernatants and frozen ampules containing twice subcloned hybridoma cell lines to the investigator and up to 20 ml hybriodma supernatant. In most projects are completed within 4 to 6 months providing the first fusion was successful. Mice are sacrificed at the conclusion of a successful project, unless client accepts mouse housing charges of $50/month for possible additional fusion(s).

Dr. Peter Yaciuk The Hybridoma Development Service Facility Department of Molecular Microbiology and Immunology Saint Louis University Health Sciences Center 1402 South Grand Boulevard Saint Louis, MO 63104 314-977-8890

Saint Louis University School of Medicine
Last modified on March 2, 2004, 2:23 pm