Flow Cytometry Research Core Facility

Saint Louis University School of Medicine

General Information: Mailing Address: Doisy Research Center

Director: Dr. Mike Green 1100 S. Grand (Rm 765)

Manager: Joy Eslick St. Louis, MO 63104

Analyst: Sherri Koehm

Phone #: 314-977-8877 Shipping Address: 1205 Carr Lane (DRC 765)

Email: eslickjb@slu.edu Attn: Flow Core Lab

St. Louis, MO 63104

Flow Cytometers Available:

BD Biosciences FACSCalibur: 2 lasers (488, 635 nm), up to 4-color analysis

BD Biosciences LSR II: 3 lasers (405, 488, 633 nm), up to 10-color analysis

BD Biosciences FACSCanto II: 2 lasers (488, 633 nm), up to 8-color analysis (located within a BSL-3 designated suite)

BD Biosciences FACSAria: 3 lasers (407,488,633 nm), up to 13-color analysis and high speed sorting of up to 4 populations simultaneously into plates or tubes

Other Instrumentation Available:

LINCO Luminex 100: fluorescent bead based assays ($15 per analysis)

Miltenyi AutoMACS Pro: cell selection/depletion/enrichment using magnetic labels

($15 per sample run)

Innovatis Cedex SC with AS20: automated viability (trypan blue) and cell counts

Thermo Shandon Cytospin 4: produces monolayer cell preps on glass slides

Service hours:

The facility is available M-F from 8AM to 5PM. If you require services outside of these hours, please contact us to discuss. It is advised to schedule your analysis time at least one week in advance to assure the day and time slot you prefer, particularly for long experiments. To schedule time for flow analysis, please call 977-8877. Please leave a message if you are unable to reach us immediately, and we will return your call as soon as possible.

Fee Information:

An hourly rate is charged for all cytometry services performed, including acquisition of samples and analysis. The total time used is rounded up to the next quarter hour (ie: 40 minutes of analysis = 45 minutes billed). There is never a charge for consultations or technical advice! There is no discount given to investigators that choose to run their own experiments.

*SLU Investigators not paying by IDO will be charged an additional 14% to cover overhead fees.

**Any analysis done in the BSL-3 suite will incur an additional $25 fee per use.