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Flow Cytometry Research Core Facility

Flow Cytometry Research Core Facility

Saint Louis University School of Medicine

General Information:

Director: Dr. Mike Green

Manager: Joy Eslick

Analyst: Sherri Koehm

Phone #: 314-977-8877

Email: eslickjb@slu.edu

Mailing Address:

Doisy Research Center

1100 S. Grand (Rm. 765)

St. Louis, MO  63104

Shipping Address:

1205 Carr Lane (DRC, 765)

Attn:  Flow Core Lab

St. Louis, MO  63104

Flow Cytometers Available:

 

BD Biosciences FACSCalibur: 2 lasers (488, 635 nm), up to 4-color analysis

 

BD Biosciences LSR II: 3 lasers (405, 488, 633 nm), up to 10-color analysis

 

BD Biosciences FACSCanto II:2 lasers (488, 633 nm), up to 8-color analysis (located within a BSL-3 designated suite)

 

BD Biosciences FACSAriaIIIu: 4 lasers (407, 488, 561, 633 nm), up to 13-color analysis and high speed sorting of up to 4 populations simultaneously into plates or tubes

 

Other Instrumentation Available:

 

LINCO Luminex 100:fluorescent bead based assays 

 

Miltenyi AutoMACS Pro:cell selection/depletion/enrichment using magnetic labels

 

Innovatis Cedex SC with AS20: automated viability (trypan blue) and cell counts

 

Thermo Shandon Cytospin 4: produces monolayer cell preps on glass slides


Service hours:

The facility is available M-F from 8AM to 5PM. If you require services outside of these hours, please contact us to discuss. It is advised to schedule your analysis time at least one week in advance to assure the day and time slot you prefer, particularly for long experiments. To schedule time for flow analysis, please call 977-8877. Please leave a message if you are unable to reach us immediately, and we will return your call as soon as possible.

Fee Information:

An hourly rate is charged for all cytometry services performed, including acquisition of samples and analysis. The total time used is rounded up to the next quarter hour (ie: 40 minutes of analysis = 45 minutes billed). There is never a charge for consultations or technical advice! There is no discount given to investigators that choose to run their own experiments.  Please contact the flow core for the current fee structure.

Flow Cytometry Hourly Rates Effective 12/1/12:

  Sorter Analysis
Internal Customers $97 $71
Internal Customers’ with Volume Discount 1 $80 $55
External Users – Gov’t funds, not-for-profit $146 $106
External Users – For Profit Organizations $176 $128

1  Volume discount rates are applied to Sorter Hours over 140 and Analysis hours over 200 in the fiscal year. Hours are reset to zero on July 1st.        


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