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Richard J. DiPaolo, Ph.D.


Richard J. DiPaolo, Ph.D.
Associate Professor
Molecular Microbiology & Immunology
Saint Louis University School of Medicine

Post-Doctoral Fellowship: NIAID, National Institutes of Health
Ph.D: Washington University in Saint Louis, 2002
B.A.: University of Chicago, 1995

Doisy Research Center
1100 South Grand Blvd
Office 707/Lab 757
Saint Louis, MO 63104

Office Phone 314-977-8860
Laboratory    314-977-8861
FAX             314-977-8717

Richard J. DiPaolo, PhD.

Richard J. DiPaolo, Ph.D.



My laboratory is interested in establishing mouse models of human diseases to develop strategies to suppress chronic inflammatory diseases. Successful strategies may be useful to develop new therapies for autoimmune diseases and chronic inflammatory diseases. We recently developed a mouse model of autoimmune gastritis, which involves immune-mediated destruction of the stomach. Autoimmune gastritis is a common disease in humans, and our mouse model mimics many aspects of human disease. Recently we discovered that chronic inflammation ultimately leads to the development of gastric cancer, and disease in mice shares many key features associated with gastric cancer development in humans. We are using combinations of cellular and molecular assays to understand how a subset of T cells, known as regulatory T cells (Tregs) affects the various stages of ongoing disease in our gastritis/gastric cancer model. Furthermore, we are identifying cytokinesthat play an important role in regulating the development of gastritis and its progression to gastric cancer. Some of these cytokines act on immune cells, and regulate inflammation, and some of these cytokines act on gastric epithelial cells, and regulate their potential to become cancer cells. We are interested in both of these activities and are investigating strategies to manipulate the activities of Tregs and cytokines to suppress chronic inflammation and to reduce the risk of cancer.

We are also studying immune responses to vaccines and infections. We currently have an ongoing project to examine the T and B cell responses to vaccine and infections. We use high-throughput sequencing technology to identify receptors expressed by T and B cells responding to vaccines and infections. We refer to this as “immune profiling” receptors responding to vaccines and infections, and we are currently immune profiling receptors in humans and in ‘humanized mice’. Our goal in this project is to understand which immune receptors are used to recognize different vaccines and infectious agents.

Lab Members

Russ Kesman Long Nguyen Jeremy Herzog Tom Makhlouf




Chauhan AK, Chen C, Moore TL, DiPaolo RJ.
Induced Expression of FCγRIIIa on CD4+ T cells Triggers Generation of IFN-γhigh Subset.The Journal of Biological Chemistry, accepted for publication January 5, 2015.  PMID: 25556651

Nguyen TL, Makhlouf NT, Kesman RA, Anthony B, Teague RM, DiPaolo RJ. Antigen specific TGF-β-induced regulatory T cells suppress late stages of autoimmune gastritis. PLOS One, August 13, 2014. PMID: 25119105


Nguyen TL and DiPaolo R. A new mouse model of inflammation induced gastritis cancer. Oncoimmunology, October, 2013. PMID:24498543


Buchwald ZS, Kiesel JR, DiPaolo R, Yang C, Novack DV, Aurora R. Osteoclast-induced Foxp3+CD8+ T-Cells limit bone loss in mice. Bone, September2013. PMID: 23756229 

Nguyen, TL, Khurana, SS, Bellone, CJ, Capoccia, BJ, Sagartz, JE, Kesman, RA, Mills, JC, DiPaolo, RJ.  Autoimmune gastritis mediated by CD4+ T cells promotes the development of gastric cancer.  Cancer Research, 2013, Feb. 1. Epub ahead of print.  PMID:  23378345

Berrien-Elliott MM, Jackson SR, Meyer JM, Rouskey CJ, Nguyen TL, Yagita H, Greenberg P, DiPaolo RJ, Teague RM. Durable adoptive immunotherapy for leukemia produced by manipulation of multiple regulatory pathways of CD8+ T-cell tolerance.  Cancer Research, 2013; 73(2):605-16.PMID:  23188506

Li JY, Adams J, Calvi LM, Lane TF, DiPaolo R, Weitzmann MN, Pacifici R.  PTH expands short-term murine hemopoietic stem cells through T cells.   Blood. 2012 November 22;120(22):4352-62. PMID:  22955916

Buchwald, ZS, Kiesel, JR, DiPaolo, RJ, Pagadala MS, and Aurora, R.  Osteoclast activated FoxP3+ CD8+ T-cells suppress bone resorption.  PLoS ONE.  May 2012 7(6): e381990.  PMID:  22701612

Thanh-Long M. Nguyen, Nicole L. Sullivan, Mark Ebel, Ryan M. Teague, and Richard J. DiPaolo. Antigen-Specific TGF-β–Induced Regulatory T Cells Secrete Chemokines, Regulate T Cell Trafficking, and Suppress Ongoing Autoimmunity.  The Journal of Immunology, 2011 187:1745-1753.  PMID:  21746962

Huter EN, Stummvoll GH, DiPaolo RJ, Glass DD, Shevach EM.  Pre-differentiated Th1 and Th17 effector T cells in autoimmune gastritis: Ag-specific regulatory T cells are more potent suppressors than polyclonal regulatory T cells.  International Immunopharmacology, 2009 May;9(5):540-5. PMID: 19539565

DiPaolo RJand Shevach EM.  CD4+ T cell-development in a mouse expressing a transgenic TCR derived from a Treg.  The European Journal of Immunology, 39(1)234-40, Jan, 2009. PMID: 19065648

Huter EN, Stummvoll GH, DiPaolo RJ, Glass DD, Shevach EM.  Cutting Edge: Antigen specific TGF beta-induced T cells suppress Th17-mediated autoimmune disease.  The The Journal of Immunology, 181(12):8209-13, Dec 15, 2008.  PMID: 1905237

Levin D, DiPaolo RJ, Brinster C, Revilleza MJ, Boyd LF, Teyton L, Natarajan K, Mage MG, Shevach EM, Margulies DH.  Availability of autoantigenic epitopes controls the phenotype, severity, and penetrance in TCR Tg autoimmune gastritis. European Journal of Immunology, 38(12):3339-53, Dec 1, 2008.  PMID:  19039784

Shevach EM, Davidson TS, DiPaolo RJ, Andersson J. Role of TGF-beta in the induction of FoxP3 expression and T regulatory cell function. Journal of Clinical Immunology, 2008 Nov;28(6):640-6.  Pubmed abstract link: 18810612

Stummvoll GH, DiPaolo RJ, Huter EN, Davidson TS, Glass D, Ward JM, Shevach EM.  Th1, th2, and th17 effector T cell-induced autoimmune gastritis differs in pathological pattern and in susceptibility to suppression by regulatory T cells. The Journal of  Immunology, 2008 Aug 1;181(3):1908-16.  Pubmed Abstract link: 18641328

DiPaolo RJ, Brinster C, Davidson TS, Andersson J, Glass D and Shevach EM.  Autoantigen-Specific TGFbeta-Induced Foxp3+ Regulatory T Cells Prevent Autoimmunity by Inhibiting Dendritic Cells from Activating Autoreactive T Cells  The Journal of  Immunology 2007 179:4685-4693.  Pubmed Abstract link: 17878367

Davidson TS, DiPaolo RJ, Andersson J, Shevach EM. Cutting Edge: IL-2 is essential for TGF-beta-mediated induction of Foxp3+ T regulatory cells. The Journal of  Immunology, 2007 Apr 1;178(7):4022-6.  Pubmed Abstract link: 17371955

Zhao DM, Thornton AM, DiPaolo RJ, Shevach EM.  Activated CD4+CD25+ T cells selectively kill B lymphocytes.  Blood, 2006 May 15;107(10):3925-32.  Pubmed Abstract link: 16418326

DiPaolo RJ, Glass DD, Bijwaard KE, Shevach EM.  CD4+CD25+ T cells prevent the development of organ-specific autoimmune disease by inhibiting the differentiation of autoreactive effector T cells. The Journal of  Immunology, 2005 Dec 1;175(11):7135-42. Pubmed Abstract link: 16301616

Haeryfar SM, DiPaolo RJ, Tscharke DC, Bennink JR, Yewdell JW.  Regulatory T cells suppress CD8+ T cell responses induced by direct priming and cross-priming and moderate immunodominance disparities.  J Immunol. 2005 Mar 15;174(6):3344-51. Pubmed Abstract link: 15749866

Research Grants


American Gastroenterological Association Pilot Research Award
Title: Determining the Effects of Gastritis on the Gastric Microbiome
Role: Principal Investigator
Period: 07/14 – 06/15

NIH-RO1 AR064821
Title: A Negative Feedback Loop Between Osteoclasts and CD8+ T cells
Role: Co-Investigator
Period: 04/14 – 03/19

American Cancer Society 22720-RSG-12-171-01-LIB
Title: The Regulation of Metaplasia in a Model of Autoimmune Gastritis
Role: Principal Investigator
Period: 07/12 – 06/16

NIH-RO1 AI098114-01
Title: T cell Activation by Immune Complexes and Complement in Autoimmunity
Role: Key Person
Period: 06/12 – 05/17

NIH-RO1 AR54625
Title: The role of PTH in T cell mediated bone metabolism
Role: Co-Investigator
Period: 09/12 – 08/17

Central Intelligence Agency HDTRA1-12C-0051
Title: Immunological Profiling to Distinguish Infection from Vaccination
Role: Co-Investigator
Period: 09/12 – 08/15


Saint Louis University President's Research Fund
Title: A novel mouse model to measure vaccine specific T effector and T regulatory responses
Role: Principle Investigator
Period: 03/0/2013 – 07/30/2014

Washington University - Digestive Disease Research Core Center Grant (P30 DK52574)
Title: The Roles of IL-27/IL-35 in Inflammation Induced Gastric Epithelial Cell Changes
Role: Principle Investigator
Period: 05/01/2012 – 04/30/2013

Saint Louis University Medical School Seed Grant
Title: The Role of IL-35 in Treg-Mediated Suppression of Arthritis
Role: Principle Investigator
Period: 05/01/2011 – 05/30/2012

Arthritis National Research Foundation
Title: Adoptively Transferring GPI-specific Regulatory T Cells to Treat the Effector Phase of Arthritis
Role: Principle Investigator
Period: 06/01/2010 – 05/30/2011

Saint Louis University President's Research Award
Title: Large scale sequence analysis to determine the extent of overlap between T cell receptors expressed by T regulatory cells and non-regulatory cells.
Role: Principle Investigator
Period: 02/01/2010 – 02/20/2011

Arthritis National Research Foundation
Title: Adoptively Transferring GPI-specific Regulatory T Cells to Treat the Effector Phase of Arthritis
Role: Principle Investigator
Period: 06/01/2009 – 05/30/2010